LOEWE Center for
Insect Biotechnology
& Bioresources

Peter Czermak

Prof. Dr.-Ing.

  • Managing Director of the Institute of Bioprocess Engineering and Pharmaceutical Technology IBPT at the Technischen Hochschule Mittelhessen
  • Adjunct Professor in Chemical Engineering at the Kansas State University, Manhattan, KS, USA
  • Professor h.c. at the Faculty 08 - Biology and Chemistry - at the Justus-Liebig University Giessen
  • Vice-head of the Fraunhofer project group "Bio-Ressources" of the Fraunhofer Institute of Molecular Biology and Applied Ecology (IME)

Phone: +49 641 309-2551

Email: peter.czermak@kmub.thm.de

Room: D10.104

Building: THM

Vita

since 2013 Vice-head of the Fraunhofer project group "Bio-Ressources" of the Fraunhofer Institute of Molecular Biology and Applied Ecology
since 2011 Professor h.c. at the Faculty 08 - Biology and Chemistry - at the Justus-Liebig University Giessen

2008–2013

Coordinator and spokesperson of the LOEWE Priority Program "Bioengineering & Imaging" at the University of Applied Sciences Giessen
since 2004 Professor in Chemical Engineering (adjunct) at the Faculty of Engineering, Department of Chemical Engineering, Kansas State University, Manhattan, Kansas, USA
1994–2000 Managing director of the Biotechnologie Gesellschaft Mittelhessen mbH, Giessen (BIM)
since 1991 Professor of Bioprocess Engineering, Membrane Technology and Cell Culture Technology at the University of Applied Sciences Giessen, Department of Biotechnology
1990 PhD (Dr.-Ing.) Bioprocess Engineering Technical University of Munich / Fraunhofer Institute for Process engineering and Packaging IVV Freising, Germany
1986–1991 applied research at the chemical/pharmaceutical industry (Akzo Nobel AG) in the field of Bioprocess Engineering and Membrane Technology; industrial experience in the field of Bioprocess Engineering and in the production of industrial fibers
1978–1984 MSc (Dipl.-Ing.) Chemical Engineering/Biochemical Engineering, University of Stuttgart and Technical University of Dortmund, Germany

Research Interests

  • Development of processes for the fermentative production of raw materials and functional food ingredients
  • Development of Process Analytical Technology (PAT) and process automation for fermentation and biocatalysis
  • Membranes and membrane-bioreactor-systems for the separation and production of biomolecules, organic substances and virus particles
  • Development of cellular and viral bioprocesses

https://www.thm.de/lse/fachbereich/team/professoren/profile/90-peter-czermak.html

Patents

EP2473594 (A2) ― 2012-07-11
CZERMAK PETER [DE]; FREIMARK DENISE [DE]; GRACE PABLO PINO [DE]; JUSTICE CHRISTIANE [DE]; WEBER CHRISTIAN [DE]
Device and method for the expansion, harvesting and differentiation of stem cells.


Publications

Grein TA, Loewe D, Dieken H, Salzig D, Weidner T, Czermak P (2018) High titer oncolytic measles virus production process by integration of dielectric spectroscopy as online monitoring system Biotechnology and Bioengineering, in press
Leber J, Barekzai J, Blumenstock M, Pospisil B, Salzig D, Czermak P (2017 (in press)) Microcarrier choice and bead-to-bead transfer for human mesenchymal stem cells in serum-containing and chemically defined media. Process Biochemistry, 59 (B) , 255-265.
Elseberg C, Leber J, Weidner T, Czermak P (2017) The Challenge of Human Mesenchymal Stromal Cell Expansion: Current and Prospective Answers InTech Open,
Grein TA, Schwebel F, Kress M, Loewe D, Dieken H, Salzig D, Weidner T, Czermak P (2017) Screening different host cell lines for the dynamic production of measles virus Biotechnology Progress, 33 (4) , 989-997.
Grein TA, Weidner T, Czermak P (2017) Concepts for the Production of Viruses and Viral Vectors in Cell Cultures InTech Open,
Sprick G, Weidner T, Salzig D, Czermak P (2017) Baculovirus-induced recombinant protein expression in human mesenchymal stromal stem cells: A promoter study. New Biotechnology, 39 (B) , 161-166.
Busse N, Czermak P (2016) Role and application of versatile peroxidase (VP) for utilizing lignocellulose in biorefineries. , in Biofuel and Biorefinery Technologies, Gupta VK, Tuohy MG (Eds.) Springer: Heidelberg, Accepted
Busse N, Fuchs F, Kraume M, Czermak P (2016) Treatment of enzyme-initiated delignification reaction mixtures with ceramic ultrafiltration membranes: Experimental investigations and modeling approach. Separation Science and Technology, 51 (9) , 1546–1565.
Busse N, Kraume M, Czermak P (2016) Optimal permeate flux for an enzymatic oxidation of technical lignins in a membrane reactor. Separation Science and Technology, Accepted
Ebrahimi M, Busse N, Kerker S, Schmitz O, Hilpert M, Czermak P (2016) Treatment of the Bleaching Effluent from Sulfite Pulp Production by Ceramic Membrane Filtration. Membranes, 6 (1) , 1–15.
Fan R, Ebrahimi M, Quitmann H, Aden M, Czermak P (2016) An Innovative Optical Sensor for the Online Monitoring and Control of Biomass Concentration in a Membrane Bioreactor System for Lactic Acid Production. Sensors, 16 (3) , 1–12.
Fan R, Ebrahimi M, Quitmann H, Czermak P (2016) Lactic acid production in a membrane bioreactor system with thermophilic Bacillus coagulans: online monitoring and process control using an optical sensor. Separation Science and Technology, Accepted
Grein TA, Leber J, Blumenstock M, Petry F, Weidner T, Salzig D, Czermak P (2016) Multiphase mixing characteristics in a microcarrier-based stirred tank bioreactor suitable for human mesenchymal stem cell expansion. Process Biochemistry, 51 (9)
Köster N, Schmiermund A, Grubelnig S, Leber J, Ehlicke F, Czermak P, Salzig D (2016) Single-Step RNA Extraction from Different Hydrogel-Embedded Mesenchymal Stem Cells for Quantitative Reverse Transcription–Polymerase Chain Reaction Analysis. Tissue Engineering Part C: Methods, 22 (6) , 552–560.
Moghaddam MR, Tonk M, Schreiber C, Salzig D, Czermak P, Vilcinskas A, Rahnamaeian M (2016) The potential of the Galleria mellonella innate immune system is maximized by the co–presentation of diverse antimicrobial peptides. Biological Chemistry, epub ahead of print
Petry F, Smith JR, Leber J, Salzig D, Czermak P, Weiss ML (2016) Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use. Stem Cells International, 2016 (ID 4834616) , 1–12.
Salzig D, Leber J, Merkewitz K, Lange MC, Köster N, Czermak P (2016) Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium. Stem Cells International, 2016 (ID 5246584) , 1–10.
Spohner SC, Schaum V, Quitmann H, Czermak P (2016) Kluyveromyces lactis: An emerging tool in biotechnology. Journal of Biotechnology, 222 , 104–116.
Ur Rehman A, Kovacs Z, Ebrahimi M, Quitmann H, Czermak P (2016) Enzymatic production of fructo-oligosaccharides from inexpensive and abundant substrates using a membrane reactor system. Separation Science and Technology, 51 (9) , 1537–1545.
Awungacha-Lekelefac C, Busse N, Herrenbauer M, Czermak P (2015) Photocatalytic based degradation processes of lignin derivatives Photochemistry Reviews, , 1–18.
Druzinec D, Salzig D, Kraume M, Czermak P (2015) Micro-bubble Aeration in Turbulent Stirred Bioreactors: Coalescence Behavior in Pluronic F68 Containing Cell Culture Media Chemical Engineering Science, 126 , 160–168.
Eisenhardt M, Dobler D, Schlupp P, Schmidts T, Salzig M, Vilcinskas A, Salzig D, Czermak P, Keusgen M, Runkel F (2015) Development of an insect metalloproteinase inhibitor drug carrier system for application in chronic wound infections Journal of Pharmacy and Pharmacology , 67 (11) , 1481-1491.
Elseberg CL, Salzig D, Czermak P (2015) Bioreactor expansion of human mesenchymal stem cells according to GMP requirements Stem Cells and Good Manufacturing Practices , 1283 in Methods in Molecular Biology, Turksen K (Eds.) , 199–218. Springer: Berlin Heidelberg
Fan R, Ebrahimi M, Quitmann H, Czermak P (2015) Lactic acid production in a membrane bioreactor system with thermophilic Bacillus coagulans: Fouling analysis of the used ceramic membranes. Separation Science and Technology, 50 (14) , 2177–2189.
Kuhn J, Müller H, Salzig D, Czermak P (2015) A new method for a rapid glycerol determination during microbial fermentation Electronic Journal of Biotechnology, 18 (3) , 252–255.
Müller H, Salzig D, Czermak P (2015) Considerations for the process development of insect-derived antimicrobial peptide production Biotechnology Progress, 31 (1) , 1–11.
Spohner SC, Müller H, Quitmann H, Czermak P (2015) Expression of enzymes for the usage in food and feed industry with Pichia pastoris. Journal of Biotechnology, 202 , 118–134.
Spohner SC, Zahn D, Schaum V, Quitmann H, Czermak P (2015) Recombinant α-L-rhamnosidase from Aspergillus terreus in selective trimming of α-L-rhamnose from steviol glycosides Journal of Molecular Catalysis B: Enzymatic, 122 , 248–254.
Weiss K, Gerstenberger J, Salzig D, Mühlebach MD, Cichutek K, Pörtner P, Czermak P (2015) Oncolytic measles viruses produced at different scales under serum-free conditions Engineering in Life Sciences, 15 (4) , 425–436.
Cierpka K, Mika N, Lange MC, Zorn H, Czermak P, Salzig D (2014) Cell detachment by prolyl-specific endopeptidase from Wolfiporia cocos American Journal of Biochemistry and Biotechnology, 10 (1) , 14–21.
Druzinec D, Weiss K, Elseberg C, Salzig D, Kraume M, Pörtner R, Czermak P (2014) Process analytical technology (PAT) in insect and mammalian cell culture processes: dielectric spectroscopy and focused beam reflectance measurement (FBRM) Methods in Molecular Biology, 1104 , 313–341.
Grein TA, Michalsky R, Czermak P (2014) Virus separation by membranes Methods in Molecular Biology, 1104 , 459–491.
Kovacs Z, Benjamins E, Grau K, Ur Rehman A, Ebrahimi M , Czermak P (2014) Recent Developments in Manufacturing Oligosaccharides with Prebiotic Functions Biotechnology of Food and Feed Additives, 143 in Advances in Biochemical Engineering/Biotechnology, Zorn H, Czermak P (Eds.) , 257–295. Springer : Berlin Heidelberg
Oppermann T,Leber J, Elseberg C, Salzig D, Czermak P (2014) hMSC production in disposable bioreactors in compliance with cGMP guidelines and PAT American Pharmaceutical Review,
Wendel SO, Perera AS, Pfromm PH, Czermak P, Bossmann SH (2014) Fermentation Optimization of Mycobacterium smegmatis Using Experimental Design British Journal of Applied Science & Technology, 4 (10) , 1472–1484.
Busse N, Wagner D, Kraume M, Czermak P (2013) Reaction kinetics of versatile peroxidase for the degradation of lignin compounds American Journal of Biochemistry and Biotechnology, 9 (4) , 365–394.
Cierpka K, Elseberg CL, Knut N, Kassem M, Salzig D, Czermak P (2013) hMSC production in disposable bioreactors with regards to GMP and PAT Chemie Ingenieur Technik, 85 (1-2) , 67–75.
Druzinec D, Salzig D, Brix A, Kraume M, Vilcinskas A, Kollewe C, Czermak P (2013) Optimization of Insect Cell Based Protein Production Processes - Online Monitoring, Expression Systems, Scale-Up Advances in Biochemical Engineering/Biotechnology, 136 , 65–100.
Gebauer E, Goßla E, Kwas C, Salzig D, Schmiermund A, Czermak P, Fuchsbauer HL (2013) Identification of transglutaminase substrates from porcine nucleus pulposus as potential amplifiers in cross-linking cell scaffolds Biomacromolecules, 14 (5) , 1564–1571.
Grein TA, Zoltan K, Ebbrahimi M, Michalsky R, Czermak P (2013) Membrane supported virus separation from biological solutions Chemie Ingenieur Technik, 85 (8) , 1183–1192.
Grumbach C, Czermak P (2013) Sichere Abluftfiltration an Laborautoklaven - Integrierter Wasserintrusionstest zur Erfüllung der Vorgaben im Bereich der Biotechnologie Filtrieren & Separieren, 27 (6) , 368–373.
Kovacs Z, Benjamins E, Grau K, Rehman AU, Ebrahimi M, Czermak P (2013) Recent Developments in Manufacturing Oligosaccharides with Prebiotic Functions Advances in Biochemical Engineering/Biotechnology, 143 , 257–295.
Quitmann H, Fan R, Czermak P (2013) Acidic organic compounds in beverage, food and feed production Biotechnology of Food and Feed Additives, 143 in Advances in Biochemical Engineering/Biotechnology, Zorn H, Czermak P (Eds.) , 91–141. Springer : Berlin Heidelberg
Salzig D, Schmiermund A, Grace PP, Elseberg C, Weber C, Czermak P (2013) Enzymatic detachment of therapeutic mesenchymal stromal cells grown on glass carriers in a bioreactor The Open Biomedical Engineering Journal, 7 , 147–158.
Schüttmann I, Bouws H, Szweda RT, Suckow M, Czermak P, Zorn H (2013) Induction, characterization, and heterologous expression of a carotenoid degrading versatile peroxidase from Pleurotus sapidus Journal of Molecular Catalysis B: Enzymatic,
Weiss K, Salzig D, Röder Y, Gerstenberger J, Mühlebach MD, Cichutek K, Pörtner R, Czermak P (2013) Influence of process conditions on measles virus stability American Journal of Biochemistry and Biotechnology, 9 (3) , 243–254.
Ebrahimi M, Kovacs Z, Schneider M, Mund P, Bolduan P, Czermak P (2012) Multistage filtration process for efficient treatment of oil-field produced water using ceramic membranes Desalination and Water Treatment, 42 (1–3) , 17–23.
Elseberg CL, Justice C, Leber J, Salzig D, Wallrapp C, Kraume M, Kassem M, Czermak P (2012) Microcarrier-based expansion process for hMSCs with high vitality and undifferentiated characteristics. The International Journal of Artificial Organs, 35 (2) , 93–107.
Grein T, Michalsky R, Vega Lopez M, Czermak P (2012) Purification of a recombinant baculovirus of AcMNPV by ion exchange membrane chromatography Journal of Virological Methods, 183 (2) , 117-124.
Paulen R, Foley G, Fikar M, Kovacs Z, Czermak P (2012) Optimal feeding strategy of diafiltration buffer in batch membrane processes Journal of Membrane Science, 411–412 , 160–172.
Roman A, Vatai G, Ittzes A, Kovacs Z, Czermak P (2012) Modeling of diafiltration processes for demineralization of acid whey: an empirical approach Journal of Food Process Engineering, 35 (5) , 708–714.
Weiss K, Salzig D, Mühlebach MD, Cichutek K, Pörtner R, Czermak P (2012) Key parameters of measles virus production for oncolytic virotherapy. American Journal of Biochemistry and Biotechnology, 8 (2) , 81-98.
Freimark D, Sehl C, Weber C, Hudel K, Czermak P, Hofmann N, Spindler R, Glasmacher B (2011) Systematic parameter optimization of a Me2SO- and serum-free cryopreservation protocol for human mesenchymal stem cells. Cryobiology, 63 (2) , 67–75.
Justice C, Brix A, Freimark D, Kraue M, Pfromm P, Eichenmueller B, Czermak P (2011) Process Control in Cell Culture Technology Using Impedance Spectroscopy. Biotechnology Advances, 29 (4) , 391–401.
Justice CL, Leber J, Freimark D, Pino Grace P, Kraume M, Czermak P (2011) Online- and offline- monitoring of stem cell expansion on microcarrier Cytotechnology, 63 (4) , 325–335.
Kovács Z, Román A, Vatai G, Ittzés A, Grachten M, Czermak P (2011) Experimental and numerical investigations on whey desalination with nanofiltration Food Industry, 22 (1) , 3–7.
Paulen R, Fikar M, Kovacs Z, Czermak P (2011) Process optimization of diafiltration with time-dependent water adding for albumin production Chemical Engineering and Processing: Process Intensification 50, , 815-821.
Paulen R, Foley G, Fikar M, Kovacs Z, Czermak P (2011) Minimizing the process time for ultrafiltration/diafiltration under gel polarization conditions J Membrane Sci 380, , 148-154.
Salzig D, Schmiermund A, Gebauer E, Fuchsbauer HL, Czermak P (2011) Influence of porcine intervertebral disc matrix on stem cell differentiation Journal of Functional Biomaterials 2, , 155-172.
Salzig D, Schmiermund A, Gebauer E, Fuchsbauer L, Czermak P (2011) Influence of porcine nucleus pulposus extract on differentiation of human mesenchymal stem cells for intervertebral disc regeneration Biomaterialien 12, , 197.
Czermak P, Catapano G (2010) You can look (but you better not touch) - Bioengineering and Imaging the international Journal o Artificial Organs, 33 (4) , 191-192.
Czermak P, Ebrahimi M, Czermak S, Catapano G (2010) Keramische Ultra- und Nanofiltrationsmembranen zur Endotoxin-Abtrennung aus Dialysierflüssigkeit und Dialysat mt-Medizintechnik 130, , 20-24.
Ehlicke F, Freimark D, Heil B, Dorresteijn A, Czermak P (2010) Intervertebral Disc Regeneration: Influence of growth factors on differentiation of human mesenchymal stem cells (hMSC) Int J Artif Organs, , 244-252.
Feimark D, Ehlicke F, Czermak P (2010) The need of imaging methods for bioengineering of three dimensional cell cultures Int J Artif Organs, , 193-197.
Fikar M, Kovacs Z, Czermak P (2010) Dynamic optimization of batch diafiltration processes Journal of Membrane Science 355, , 168-174.
Freimark D, Grace PP, Weber C, Pohl S, Poertner R, Wallrapp C, Geigle P, Czermak P (2010) Use of encapsulated stem cells to overcome the bottleneck of cell availability for cell therapy approaches Transfusion Medicine and Hemotherapy 37, , 66-73.
Grein T, Freimark D, Weber C, Hudel K, Wallrapp C, Czermak P (2010) Alternatives to dimethylsulfoxide for serum-free cryopreservation of human mesenchymal stem cells Int J Artif Organs, , 370-380.
Michalsky R, Passarelli AL, Sorensen CM, Pfromm PH, Czermak P (2010) Concentration of the Baculovirus Autographa californica M Nucleopolyhedrovirus (AcMNPV) by Ultrafiltration Desalination 250, , 1125-1127.
Weber C, Pohl S, Poertner R, Grace PP, Freimark D, Wallrapp C, Geigle P, Czermak P (2010) Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system – Part B: Modeling and scale up Int J Artif Organs, , 782-795.
Weber C, Pohl S, Poertner R, Grace PP, Freimark D, Wallrapp C, Geigle P, Czermak P (2010) Expansion of human mesenchymal stem cells in a fixed-bed bioreactor system – Part A: Inoculation, cultivation, cell harvest procedures Int J Artif Organs, , 512-525.
Weber C, Pohl S, Poertner R, Grace PP, Freimark D, Wallrapp C, Geigle P, Czermak P (2010) Production process for stem cell-based therapeutic implants - Expansion of the production cell line and cultivation of encapsulated cells Advances in Biochemical Engineering/Biotechnology 123, , 143-162.
Weber C, Sehl C, Grein T, Hudel K, Geigle P, Czermak P (2010) Konservierung von zelltherapeutischen Produkten – Einfrieren nach GMP GIT Laborfachzeitschrift, , 768-770.